X-ray Microtomograph Skyscan 1172 80kV
Bruker mCT, Kontich, Belgium
DESCRIPTION OF THE INSTRUMENT:
- X-ray source: Hamamatsu L6732, 20-80kV, 100µA, 8W, spot size <8µm
- X-ray detector: Ximea 11Mp (4000×2670) 12-bit CCD
- Maximum sample size : 50mm diameter, 70mm height
- Detail detectability : 0.5-27µm
- Skyscan software suite (Control, Reconstruction, Measurement, Visualization)
As a non-destructive technique, X-ray microtomography is the best choice to investigate hard tissue in volumes at a micrometric scale. Softer tissue might need a specific staining in order to contrast your volume of interest. In our laboratory we use osmium teroxyde staining to show adiposity contained in the bone marrow under X-ray illumination. It is possible to use Polymethyl Metacrylate resin to embed bone samples for long term conservation and easy manipulation. Softer samples are scanned with adapted tubes and holding medium.
DATA TREATMENTS & ANALYSIS:
As a tridimensionnal analysis technique, X-ray microtomography provides a quantitative analysis as measurements of volumes. Usual measurements correspond to 2D and 3D morphometry analysis. It’s also possible to do qualitative analysis with adapted calibration phantoms. In our laboratory we use Calcium Hydroxyapatite phantoms to do bone mineral density evaluation.
X-ray microtomography provides projections of the sample within an acquisition dataset. This dataset is used to reconstruct the volume as cross-sections. Treatment is done on cross-sections to determine a volume of interest and segmentation by binarization is used to provide the volume which will be analysed. Vizualisation might be done as multi-planar and volume or surface rendering.